Kibron MicroTroughX allows
for excellent reproducibility
in surface chemistry
experiments.

"While using Kibron MicroTroughX to complete a major part of my PhD thesis I was impressed with both its ease-of-use and the quality and reproducibility of the measured data. I've continued to use the instrument ever since as a part of my research work".

 

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Kibron MicroTroughXL - unmatched reproducibility to your experiments

The multipurpose Kibron MicroTroughXL allows for unmatched reproducibility in surface chemistry experiments, as demonstrated below for two consecutive compression and surface potential isotherms for ceramide monolayer (courtesy of Dr. Karen Sabatini). 

 

 

 

 

 

 

MicroTroughXL (equipped with a Precision Plus trough) was used to simultaneously measure -A and Δψ-A isotherms, using the embedded features of the FilmWare software.

Aliquot of the lipid (in chloroform) was spread onto the air-aqueous phase (10 mM phosphate saline buffer, 1 M NaCl, pH 6.6) interface with a microsyringe. Total surface area of the trough is 230 cm2 and the volume of the subphase 40 ml.

After 5 min equilibration (to ensure evaporation of the solvent) film compression was started using two symmetrically moving barriers. Compression rate was 4 Å2/chain/min, so as to allow for the reorientation and relaxation of the lipids in the course of the compression.

Surface pressure () was monitored with a Kbn probe hanging from the Kibron high precision microbalance (KBN 315), connected to a computer. Monolayer surface potential ψ was measured using the vibrating plate method (Kibron μSpot). Both isotherms were recorded at ambient temperature.

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MicroTroughXL

The MicroTroughXL is equipped with an extended trough for increased precision of experiments and reproducibility.