LB films, compression isotherms and many other applications

Biomembranes, membrane ‘rafts’, lipids, and membrane proteins are entering into the forefront of biosciences. Use of monolayers has paved way to breakthroughs in the molecular mechanisms of action of phospholipases, lung surfactants, cellular signaling, antibiotic peptides, blood coagulation, and cell adhesion, just to name a few examples.

The Kibron MicroTroughX and its accessories are ideal for the following wide range of applications:

• penetration of peptides and proteins into lipid monolayers

• 2-D crystallization of proteins

• action of enzymes in monolayers recording of surface pressure, isotherms, isobars, and isochors

• studying the organization of lipid rafts

• measuring interactions of drugs, peptides and proteins with lipids

• fluorescence microscopy and spectroscopy of monolayers

• monitoring enzyme action on monolayers

• determination of the surface activity and CMC of drugs

• surface potential studies

• deposition of Langmuir Blodgett film / LB film on EM-grids and mica for AFM studies

• X-ray and neutron reflectivity studies

• FTIR-measurements

• Brewster-angle microscopy

• ellipsometry

• complex formation by nucleic acids and cationic lipids

• surface tension measurements

• spectroscopic measurements

Compression
isotherms with
MicroTroughX