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DeltaPiSingle- and 4-channel Langmuir tensiometer for the measurement of binding of drugs, peptides, and proteins to lipid membranes. You can request a quotation for a Kibron DeltaPi by pressing either of the links below:
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Overview
Binding/intercalation of drugs and peptides/proteins into lipid membranes...
Biomembranes, membrane ‘rafts’, lipids, and membrane proteins are entering into the forefront of biosciences. Use of monolayers has paved way to breakthroughs in the molecular mechanisms of action of phospholipases, lung surfactant, cellular signaling, antibiotic peptides, blood coagulation, and cell adhesion, just to name a few examples.
The Kibron DeltaPi and DeltaPi-4 can be readily operated as standard, high precision tensiometers. However, they can be operated as Langmuir-tensiometers and arethus ideal for the following applications:
monitoring enzyme action on monolayers
2-D crystallization of proteins
measuring specific interactions of drugs and peptides/proteins with lipid monolayers
complex formation by nucleic acids (DNA, siRNA) and cationic lipids
A large number of experiments can be made with Kibron DeltaPi/DeltaPi-4, with minimal material consumption. One milligram of a precious peptide is plenty when you work with DeltaPi.
Specifications
| Base mechanics | 33 x 14 x 6 (h) cm ( 13 x 5.5 x 2.4 inches) 0.85 kg ( 1.8 lbs) |
| Electronics | 27 x 23 x 8 cm (10.6 x 9 x 3.1 inches) |
| Total weight | 2.8 kg (5.9 lbs) |
Measurement of surface tension and surface pressure
| Principle |
Wilhelmy method using a small diameter ( 0.51 mm) special alloy wire. No artefactual hysteresis as when using the conventional Pt-plates. No drying and accumulation of salt which cause significant errors when filter paper is used. |
| Dynamic range | 0 to 130 mN/m |
| Resolution | 10 µN/m |
| Temperature sensor | Automatic monitoring and display of temperature in the course of the experiment, resolution 0.1 degrees (optional feature) |
Troughs
| Materials | Metal alloy and high purity PTFE. Several designs available. Also user-defined designs are routinely made. |
| Volume | 300 µl in surface pressure measurements. Subphase can be magnetically stirred. |
Accessories
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Butane torchButane torch for cleaning the DyneProbes. The torch is a GB-2001, manufactured by Prince. |
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DyneProbeChemically resistant. Made for good surface wettability. Du Noüy-Padday technique. Suitable probe for small sample volume. |
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DyneProbe handling toolTool for safe and easy cleaning, adding and removal of DyneProbes from AquaPi, EZPiplus, MTXS, MTXL, DeltaPi, and DeltaPi-4. |
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Probe maintenance stationIdeal for storing and maintaining DyneProbes, in particular when used with DyneProbe handling tool. |
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Temperature sensorSensor for accurately measuring the temperature of the measured substance. |
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Multiwell plateGlass bottom multiwell plate with Teflon rims. Ideal for penetration measurements, when availability of materials is limited, with subphase volume of only 300 microliters, optionally with magnetic stirring. |
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Multiwell plate, Aluminium, Teflon coatedTeflon coated Aluminium multiwell plate, with injection ports, for penetration measurements. Optional with magnetic stirrers. Subphase volume 1.3 ml. |
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All Teflon multiwell plate
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Publications
Publications
Click here to see an assorted list of publications utilizing Kibron DeltaPi or Kibron DeltaPi-4.
Customer Testimonials
"Since the very early ‘90’s, our research team led by Professor Christian Salesse has been interested in the study of protein-lipid interaction using various commercial and custom-made Langmuir throughs.
In recent years, we acquired two Kibron Delta-Pi microthrough systems as well as their multiwell plates, and we have been very satisfied by their quality, simplicity and ease of use.
The only match to the excellence of their products is their outstanding after sale service."